qubit®(早期称为Quant-iT™)dsDNAHS分析试剂盒(Qubit®dsDNAHSAssayKit)专门设计用于在Qubit®3.0荧光仪(Q32866),但也可以在任何荧光计或荧光酶标仪中使用。该试剂盒对双链DNA(dsDNA)有高度的选择性，不会定量RNA，可以定量浓度为10pg/µl到100ng/µl的样品。试剂盒提供了浓缩的分析试剂，稀释缓冲液和预制的DNA标准。您只需用提供的缓冲液稀释试剂，添加样品(体积在1µL到20µL范围内),然后使用Qubit®3.0荧光仪读取浓度。该试剂盒对于常见污染物，如盐，游离的核苷酸，溶剂，去污剂，蛋白质的耐受性极好。
注意:所有的Qubit®分析试剂盒同样适用于Qubit®3.0荧光仪。
Description
TheQubitdsDNAHS(HighSensitivity)AssayKitisdesignedspecificallyforusewiththeQubitFluorometer.Theassayishighlyselectivefordouble-strandedDNA(dsDNA)overRNAandisdesignedtobeaccurateforinitialsampleconcentrationsfrom10pg/µLto100ng/µL.Thekitprovidesconcentratedassayreagent,dilutionbuffer,andpre-dilutedDNAstandards.Simplydilutethereagentusingthebufferprovided,addyoursample(anyvolumebetween1µland20µlisacceptable),andreadtheconcentrationusingtheQubitFluorometer.Commoncontaminants,suchassalts,freenucleotides,solvents,detergents,orproteinarewelltoleratedintheassay.
WhichproducttochoosefordsDNAHS(HighSensitivity)quantitation?
•For1–20samples:usethisQubitdsDNAHSAssayKitwiththeQubitFluorometer
•For20–2000samples:usetheQuant-iTdsDNAHSAssayKitwithmicroplatereader
Notes:
1.AllQubitassaykitscanbeusedwiththeQubit1.0,Qubit2.0,Qubit3,andQubit4fluorometers.
2. 500µLthin-walledPCRtubes arerequiredbutnotincluded.
3.Alsoavailableasa"1X"dsDNAassaykitthatprovidesreagentandbufferinaformulationthatisstableasaready-to-usesolutionforuptooneyearafterpreparation.
ForResearchUseOnly.Notforuseindiagnosticprocedures.
