IL-2Antibody(701080)inIHC(P)
ImmunohistochemistryanalysisofIL-2showingstaininginthecytoplasmofparaffin-embeddedhumanskintissue(right)comparedtoanegativecontrolwithoutprimaryantibody(left).Toexposetargetproteins,antigenretrievalwasperformedusing10mMsodiumcitrate(pH6.0),microwavedfor8-15min.Followingantigenretrieval,tissueswereblockedin3%H2O2-methanolfor15minatroomtemperature,washedwithddH2OandPBS,andthenprobedwithaIL-2ABfinity™RabbitMonoclonalAntibody(Product#701080)dilutedin3%BSA-PBSatadilutionof1:20overnightat4°Cinahumidifiedchamber.TissueswerewashedextensivelyinPBSTanddetectionwasperformedusinganHRP-conjugatedsecondaryantibodyfollowedbycolorimetricdetectionusingaDABkit.Tissueswerecounterstainedwithhematoxylinanddehydratedwithethanolandxylenetoprepformounting.
Product Details
TESTED APPLICATIONSDILUTION
ELISA (ELISA)1:50-1:3200
Flow Cytometry (Flow)1:100
Immunohistochemistry (Paraffin) (IHC (P))1:10-1:50
Western Blot (WB)0.5-2 ug/ml
Tested Species reactivityHuman
Host / IsotypeRabbit / IgG
ClassMonoclonal
TypeAntibody
Clone2H20L7
ImmunogenRecombinant protein corresponding to amino acids 21–153 of human Interleukin 2
ConjugateUnconjugated
FormLiquid
Concentration0.5 mg/ml
PurificationProtein A
Storage bufferPBS
Contains0.09% sodium azide
Storage conditionsMaintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
RRIDAB_2532376
Product Specific Information
ABfinity
recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptIBLe to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
701080 was successfully to detect IL-2 in Flow and ELISA application.
Background/Target Information
IL-2 is a 15 kDa cytokine which is produced by immature CD4 Th precursor and Th0 cells as well as some Th1 cells of the immune system. IL-2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The gene for IL-2 is located on chromosome number 14 at the 4q26-q27 locus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
